Candidates for seed size, oil content and seed coat colour were identified from transcriptome analysis in three different stages, of two contrasting Brassica juncea varieties.
(Reference: https://doi.org/10.1038/s41598-022-15938-5)
Candidate Genes
| Seed Trait | Brassica juncea Transcript ID | Orthologous Arabidopsis Gene ID | Gene Symbol | Function | Reference |
|---|---|---|---|---|---|
| Seed size | A04_VARUNA_g3379.t1 | AT2G46660 | ENHANCER OF DA1 (EOD3)/CYP78A6 | Loss of function mutation produced small seeds. Overexpression increased the seed size, Promotes cell expansion and proliferation of cotyledons maternally | https://doi.org/10.1111/j.1365-313X.2012.04907.x , https://doi.org/10.1016/j.pbi.2016.05.008 |
| Seed size | B05_VARUNA_g6603.t1 | AT2G46660 | ENHANCER OF DA1 (EOD3)/CYP78A6 | Loss of function mutation produced small seeds. Overexpression increased the seed size, Promotes cell expansion and proliferation of cotyledons maternally | https://doi.org/10.1111/j.1365-313X.2012.04907.x , https://doi.org/10.1016/j.pbi.2016.05.008 |
| Seed size | A04_VARUNA_g90.t1 | AT3G61830 | ARF18 | Regulates seed weight negatively through auxin response pathway, and acts maternally, Overexpression of this gene in Arabidopsis showed change in seed weight | https://doi.org/10.1073/pnas.1502160112 |
| Seed size | A09_VARUNA_g5135.t1 | AT3G61830 | ARF18 | Regulates seed weight negatively through auxin response pathway, and acts maternally, Overexpression of this gene in Arabidopsis showed change in seed weight | https://doi.org/10.1073/pnas.1502160112 |
| Seed size | B05_VARUNA_g650.t1 | AT3G61830 | ARF18 | Regulates seed weight negatively through auxin response pathway, and acts maternally, Overexpression of this gene in Arabidopsis showed change in seed weight | https://doi.org/10.1073/pnas.1502160112 |
| Seed size | B03_VARUNA_g7431.t1 | AT3G61830 | ARF18 | Regulates seed weight negatively through auxin response pathway, and acts maternally, Overexpression of this gene in Arabidopsis showed change in seed weight | https://doi.org/10.1073/pnas.1502160112 |
| Seed size | B08_VARUNA_g22.t1 | AT4G38600 | UPL3 | Codes for a HECT E3 ubiquitin ligase which regulates seed weight negatively by proteosomal degradation of LEC2 responsible for seed maturation | https://doi.org/10.1105/tpc.18.00577 |
| Seed size | A03_VARUNA_g2832.t1 | AT3G61880 | CYP78A9 | Codes for a cytochrome P450 monooxygenase CYP78A9 and regulates seed weight positively through auxin metabolism pathway, cyp78a9-KO mutants had smaller and lighter seeds than the wild type | https://doi.org/10.1111/tpj.14236 |
| Seed size | A04_VARUNA_g456.t1 | AT3G61880 | CYP78A9 | Codes for a cytochrome P450 monooxygenase CYP78A9 and regulates seed weight positively through auxin metabolism pathway, cyp78a9-KO mutants had smaller and lighter seeds than the wild type | https://doi.org/10.1111/tpj.14236 |
| Seed size | A04_VARUNA_g86.t1 | AT3G61880 | CYP78A9 | Codes for a cytochrome P450 monooxygenase CYP78A9 and regulates seed weight positively through auxin metabolism pathway, cyp78a9-KO mutants had smaller and lighter seeds than the wild type | https://doi.org/10.1111/tpj.14236 |
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