Characterized genes of Brassica juncea regulating different seed traits like seed development, seed size, oil content, etc., obtained from literature mining.
Characterized Genes Regulating Seed Traits
| Gene Symbol | Gene Name | Function | Trait | Detail | Reference |
|---|---|---|---|---|---|
| BjAGPase | ADP-glucose pyrophosphorylase | Diverts the photosynthetically fixed carbon pool from starch to lipid biosynthesis | Seed | Down-regulation of BjAGPase and the seed-specific expression of WRINKLED1 transcription factor from A. thaliana using metabolic engineering resulted in significant increase in the seed oil content | https://doi.org/10.1016/j.plaphy.2016.06.004 |
| BjFAE | Fatty Acid Elongation | Involved in the synthesis of erucic acid from oleic acid | Oil | Down-regulation of FAE1 leads to significant decrease in the erucic acid content in seed oil | https://doi.org/10.1007/s11816-007-0032-5 |
| BjFAE1 | Fatty Acid Elongation 1 | Involved in the synthesis of erucic acid from oleic acid. | Oil | Over-expression of the FAE1 gene causes 36% increase in the percent of erucic acid. The down-regulation of FAE1 caused an 86% decrease in the percent of erucic acid | https://doi.org/10.1007/s00299-005-0068-3 |
| BjMYB28 | R2R3-MYB transcription factor family gene | Involved in aliphatic glucosinolate biosynthesis | Leaf; Seed | Significant down-regulation of BjMYB28 through target gene silencing, reduce the glucosinolates in both leaves and seeds | https://doi.org/10.1111/pbi.12078 |
| Bra jI | Allergen protein | Allergen protein, 2S albumin class, one of the most abundant seed storage protein classes | Seed | CRISPR/Cas9-induced mutations leads to reduced or absent the amount of Bra jI protein in seed extracts | https://doi.org/10.1111/tpj.15584 |
| CYP79F1 | Cytochrome P450 monooxygenase 79F1 | Mapped to QTL J16Gsl4 in the LG B4 of B. juncea | Oil | Genetic and transgenic approaches confirmed CYP79F1 as a candidate gene in regulation of synthesis of sinigrin | https://doi.org/10.1371/journal.pone.0150060 |
| CYP83A1 | Cytochrome P450 monooxygenase 83A1 | ControlS the complex aliphatic glucosinolates biosynthesis | Oil | Ectopic expression confirmed involvement of CYP83A1 in the synthesis of C4 aliphatic glucosinolates | https://doi.org/10.1007/s00425-014-2205-0 |
| FAD2 | Fatty acid desaturase | Modify fatty acid composition in the seed oil | Oil | Antisense suppression of fad2 develop low polyunsaturated fatty acid lines in the ‘0’ erucic line | https://doi.org/10.1023/B:MOLB.0000034092.47934.d6 |
| G proteins | Heterotrimeric G-proteins | Regulate plant height, seed weight, silique size and pathogen response | Plant height; Seed; Silique; Biotic stress | RNAi based suppression of BjuGβ and BjuGƴ genes suggested involvement in plant height (BjuGβƴA2 and BjuGβƴC), seed weight (BjuGβGƴA1 and BjuGβGƴC), silique size (BjuGβGƴC) and pathogen response (BjuGβGƴA1 and BjuGβGƴC) | https://doi.org/10.1007/s11103-021-01165-5 |
| GSL-ALK | Glucosinolates | Catalyzes the conversion of desirable glucoraphanin to deleterious gluconapin and progoitrin | Oil | Silencing of the GSL-ALK gene family leads to high glucoraphanin accumulation. | https://doi.org/10.1038/srep18005 |
| GTR1, 2 | Glucosinolate transporters | Components of the source to sink translocation network of glucosinolates. | Biotic stress, Oil | GTR1 and GTR2 silenced plants displayed variation in accumulation of glucocinolate among B. juncea tissues. GTR2 knockdown resulted in more resistance toward Spodoptera litura. | https://doi.org/10.1111/pce.14072 |
| MAM1 | Methythioalkylmalate synthase | 3C aliphatic glucosinolate metabolic genes | Oil | Silencing of BjMAM1–4 or BjMAM1–6 reduce the content of aliphatic glucosinolate significantly | https://doi.org/10.1016/j.hpj.2020.10.001 |
| Myb28 | Controls complex glucosinolate traits in polyploid B. juncea | Oil | Polyploidy on gene structure, phylogeny, and gene expression and function studies showed that all four BjuMYB28 genes encode functional BjuMYB28 proteins regulating aliphatic glucosinolate pools. | https://doi.org/10.1093/jxb/ert280 | |
| SGT | UDP- glucose: sinapate glucosyltransferase | Involved in the final steps of sinapine biosynthetic pathway | Oil | Low sinapine mustard lines were developed through transgenic methods, targeting key genes (SGT and SCT) in the sinapine biosynthetic pathway | https://doi.org/10.1371/journal.pone.0182747 |
| SGT & SCT | Sinapoylglucose: choline sinapoyltransferase | Involved in the final steps of sinapine biosynthetic pathway | Oil | Low sinapine mustard lines were developed through transgenic methods, targeting key genes (SGT and SCT) in the sinapine biosynthetic pathway | https://doi.org/10.1371/journal.pone.0182747 |
| TT8 | Transparent testa 8 | Flavonoid biosynthesis | Seed | TT8 gene is involved in yellow-seeded trait as they inhibit the transcription of late biosynthetic genes in the yellow-seeded mutant of B. as juncea | https://doi.org/10.1007/s00122-013-2222-6 |
To submit any new characterized gene, email us the link of your publication at namrata@cuh.ac.in